Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with SEPP1. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for SEPP1. The competitive inhibition reaction is launched between with pre-coated SEPP1 and SEPP1 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of SEPP1 in the samples. The color development is stopped and the intensity of the color is measured